. Le bulletin biologique. Biologie ; zoologie ; biologie marine. Le cuivre, enzymes, et la fécondation. 93 Ces modifications deviennent intelligibles si nous supposons, dans un cas, la formation d'infimes traces de xanthate de cuivre ; dans le second, de ferrocyanure cuivre. Puisque les mêmes réactifs ne produisent pas de changements notables dans ces endroits en matériau non traité, il semble que le cortex d'un oeuf exposés à plus de cuivre contient la quantité normale du métal. En outre, l'excédent est principalement localisée près de la surface, et comme il n'est pas le roulement de l'agglutinine c'est le cuivre, mais le lipolysin, il

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. The Biological bulletin. Biology; Zoology; Marine biology. COPPER, ENZYMES, AND FERTILIZATION. 93 These changes become intelligible if we assume, in the one case, the formation of minute traces of copper xanthate; in the second, of copper ferrocyanide. Since the same reagents produce no noticeable changes in these locations in untreated material, it appears that the cortex of an egg exposed to copper contains more than the normal quantity of the metal. Moreover, the excess is localized chiefly near the surface, and since it is not the agglutinin that is copper bearing, but the lipolysin, it appears probable that the latter is also concentrated immediately under the vitelline membrane. X. Copper Map of the Normal Egg. From all these tests—those on the controls as well as those on material exposed to copper sulphate, I have constructed a chart which indicates the distribution of copper in the normal Arbacia egg. This map is a visual summary of the chief results and infer- ences, and in view of the preceding discussion seems to require no other comment than that given in the legend under the figure.. Diagram showing distribution of copper in the normal egg of Arbacia punctulata. The central clear area is the nucleus. Immediately about this, and extending to the cortex, the larger black spots represent pigment granules in which copper was demonstrated indirectly by analysis of secreted pigment and directly, in situ, as potassium-copper-lead nitrite. The latter, as well. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original work.. Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-; Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report; HighWire Press. Lancaster, Pa. [etc. ] : Lancaster Press, inc. [etc. ]